Journal: Blood Vessels, Thrombosis & Hemostasis

Article Title: Resilience of the bone marrow vascular architecture to aging and hematological stress

doi: 10.1016/j.bvth.2026.100156

Figure Lengend Snippet: The architecture of CD31 hi vessels in murine femora is highly conserved. (A) Femora from young adult naïve, aged, and blood-let mice were excised, benzoic acid benzyl ester-cleared, imaged by LSFM in stitched tile scans, and the resulting 3D data sets segmented for CD31 hi and CD105 + vasculature. (B-D) Quantitative analysis of the CD31 hi vasculature in 5 young adult male mice (n = 5). (B) Distribution of branch hierarchy levels with a Gaussian-like peak at level 14. (C) Branch length distribution peaking at ∼100 μm with extending to 3000 μm. (D) Branch diameter distribution ranging from 9 μm to 70 μm, peaking at 19 μm. (E-G) Quantitative analysis of the CD31 hi vasculature in 5 young adult female mice (n = 5), same as (B-D) for male mice. (H) 3D reconstruction of the CD31 hi vasculature for the young mice shown in panels B-G demonstrating high structural conservation. Scale bar, 1 mm. An overlay of all 5 male vessel trees is shown in .

Article Snippet: CD105 + vessels were segmented in Imaris and skeletonized to assess parameters such as vessel volume, vessel-vessel distance, branch length, and branching degree.

Techniques:

Journal: Blood Vessels, Thrombosis & Hemostasis

Article Title: Resilience of the bone marrow vascular architecture to aging and hematological stress

doi: 10.1016/j.bvth.2026.100156

Figure Lengend Snippet: CD105 + vessels exhibit conserved architecture and proximal narrowing in young mice. (A) Representative midshaft section of CD105 + vasculature (green) in a young naïve male mouse. Bottom row: fluorescence intensity, segmented surface, skeletonized tree, and the vessel-to-vessel distance map (200-μm section). Skeleton (cyan), end points (blue), and branching points (red). Gray outline indicates marrow boundary. Scale bars, 500 μm. (B-D) Distribution of (B) vessel diameter, (C) branching angle, and (D) vessel-to-vessel spacing in young naïve male (top) and female (bottom) mice (n = 5 per sex). See “Methods” and for detailed results. (E-J) Regional analyses along the femoral shaft divided into 9 overlapping tiles (see ). Distribution of local values of (E) vessel diameter, (F) branching angle, and (G) vessel-to-vessel distance in representative hip, mid, and knee regions of the shaft. The top row shows results of male mice, the bottom row of female mice. (H-J) Averaged values of (H) vessel diameter, (I) branching angle, and (J) vessel-to-vessel distance for each region: hip-proximal (yellow), midshaft (magenta), and knee-proximal (grape). Filled bars represent male mice, lighter colored shaded bars female mice. Shown are mean ± SEM. SEM, standard error of the mean.

Article Snippet: CD105 + vessels were segmented in Imaris and skeletonized to assess parameters such as vessel volume, vessel-vessel distance, branch length, and branching degree.

Techniques: Fluorescence

Journal: Blood Vessels, Thrombosis & Hemostasis

Article Title: Resilience of the bone marrow vascular architecture to aging and hematological stress

doi: 10.1016/j.bvth.2026.100156

Figure Lengend Snippet: Morphological differences in CD105 + small vessels of aged vs young mice. (A-B) Representative midshaft femur sections from an aged male (A) and a young, blood-let mouse (B), showing segmented CD105 + surfaces and skeletonized vasculature. Color coding as in . Scale bar, 500 μm. (C-E) Distribution of vessel diameters along the femoral shaft. (F-H) Mean vessel diameter per region along the femur. (I-K) Distribution of vessel-to-vessel distances. (L-N) Regional mean vessel-to-vessel distances. Dashed lines and lighter shaded bars indicate female data. See for further details.

Article Snippet: CD105 + vessels were segmented in Imaris and skeletonized to assess parameters such as vessel volume, vessel-vessel distance, branch length, and branching degree.

Techniques:

Journal: Blood Vessels, Thrombosis & Hemostasis

Article Title: Resilience of the bone marrow vascular architecture to aging and hematological stress

doi: 10.1016/j.bvth.2026.100156

Figure Lengend Snippet: Regional changes in the CD105 + microvascular complexity along the femur. (A) Fraction of long branches (>100 μm) in young naïve (blue), blood-let (red), and aged (gray) mice (filled symbols, male; open symbols, females). (B) Proportion of vessels running parallel to the central vein. (C) Fraction of vessels with diameters <25 μm. (D) Ratio of vessel segments to branching points. (E) Ratio of end points to branching points. (F) Fraction of branching points with ≥4 outgoing branches, only branches longer than 25 μm and branching degree ≥3 were included. All data shown as mean ± SEM. SEM, standard error of the mean.

Article Snippet: CD105 + vessels were segmented in Imaris and skeletonized to assess parameters such as vessel volume, vessel-vessel distance, branch length, and branching degree.

Techniques:

Journal: Blood Vessels, Thrombosis & Hemostasis

Article Title: Resilience of the bone marrow vascular architecture to aging and hematological stress

doi: 10.1016/j.bvth.2026.100156

Figure Lengend Snippet: Modest increased MK volume in aged male mice. (A) Representative midshaft section showing CD105 + MKs (magenta) in a young adult male mouse. Bottom row: fluorescence intensity, segmented surface, MK outlines, and MK-vessel distance map (200-μm section). MK outlines and distance transform shown for a single slice. Gray outline indicates marrow boundary. Scale bar, 500 μm. (B-D) Quantification of MK properties in young (blue), aged (gray), and blood-let (red) mice (filled symbols: males; open symbols: females). (B) MK-to-AF volume ratio; (C) MK surface-to-volume ratio; and (D) proportion of MK surface within 5 μm of a vessel. (E-G) MK-vessel distance distributions; dashed lines: female mice. (H-J) Regional mean MK-vessel distances for hip, mid, and knee. Lighter colored shaded bars: females. All data shown as mean ± SEM. See “Methods” and and for details. BM, bone marrow; SEM, standard error of the mean; Sur./Vol., surface/volume.

Article Snippet: CD105 + vessels were segmented in Imaris and skeletonized to assess parameters such as vessel volume, vessel-vessel distance, branch length, and branching degree.

Techniques: Fluorescence

Journal: Cells

Article Title: Alterations in Circulating Progenitor Cell Composition in Rheumatoid Arthritis

doi: 10.3390/cells15080726

Figure Lengend Snippet: CD235a + and CD45 − CD31 − stromal cells circulating in peripheral blood. ( a ) CD45/CD31 dot plot showing populations 16, 17, 18, 19 and 20. ( b ) UMAP shows the size of each cluster, and ( c ) TriMAP shows the relative cluster location. CD45/CD31 plots highlighting the expression of ( d ) CD235a, ( e ) CD133, ( f ) CD34 and ( g ) CD105. The intensity scale indicates high expression of the marker in red. Frequency of ( h ) Pop 20, ( i ) Pop 17, and ( j ) Pop 18. Mann-Whitney test. Dot plots showing the expression of ( k ) CD34/CD133, ( l ) CD271/ CD105, and CD90 with ( m ) CD105, ( n ) CD271, and ( o ) PDPN in Pop 16 and Pop 19. Frequency of ( p ) Pop 16 and ( q ) Pop 19. t -test for Pop 16; Mann–Whitney test for Pop 19. All dot plots show mean and SD, n = 12 RA and 9 HC.

Article Snippet: CD105 , VioBlue-A , 130-099-666 , Miltenyi Biotec , 1/100 , V3.

Techniques: Expressing, Marker, MANN-WHITNEY